PUBLICATIONS
*Corresponding author(s)
2022
RNA Compensation: A Positive Feedback Insulation Strategy for RNA-Based Transcription Networks
Liu,B., Samaniego, C, C., Bennett, M., Chappell, J.*, and Franco, E.*
ACS Synthetic Biology | 2022| Link
A split ribozyme that links detection of a native RNA to orthogonal protein outputs.
Gambill, L., Staubus, A., Ameruoso, A., and Chappell, J. *
Submitted | 2022 | Preprint
Uncovering the distinct properties of a bacterial type I-E CRISPR activation system
Villegas Kcam, M.V., and Chappell, J. *
ACS Synthetic Biology | 2022 | Link
2021
Activating natural product synthesis using CRISPR interference and activation systems in Streptomyces.
Ameruoso, A., Villegas Kcam, M.V., Cohen K.P., and Chappell, J. *
Submitted | 2021 | Preprint
Computational design of Small Transcription Activating RNAs (STARs)
Liu, B. and Chappell, J.*
Methods in molecular biology, submitted | 2021
Design, characterization, and application of targeted gene activation in bacteria using a modular CRISPRa system
Villegas Kcam, M.V., and Chappell, J.*
Methods in molecular biology, submitted | 2021
Rational engineering of a modular and target flexible bacterial CRISPR-Cas activation platform.
Villegas Kcam, M.V., Tsong, A. and Chappell, J.*
Nucleic Acids Research | 2021 | Link
2019
Brave new ‘RNA’ world—Advances in RNA tools and their application for understanding and engineering biological systems.
Ameruoso, A., Liu, B., Villegas Kcam, M.V., Gambill, L., Chappell, J. *
Current Opinion in Systems Biology | 2019 | Link
A synthetic system for asymmetric cell division in Escherichia coli.
Molinari, S., Shis, D.L., Bhakta, S.P., Chappell, J., Igoshin, O.A., Bennett, M.
Nature Chemical Biology | 2019 | Link
Distinct timescales of RNA regulators enable the construction of a genetic pulse generator.
Westbrook, A., Tang, X., Marhsall, R., Maxwell, C., Chappell, J., Agrawal, D., Dunlop, M., Noireaux, V., Beisel, C., Lucks, J. B., Franco, E. M.
Biotechnology and Bioengineering | 2019 | Link
PLANT-DX: A Molecular Diagnostic for Point of Use Detection of Plant Pathogens.
Verosloff, M., Chappell, J., Perry, K., Thompson, J., Lucks, J.B.
ACS Synthetic Biology | 2019 | Link
2017
Computational design of Small Transcription Activating RNAs (STARs) for versatile and dynamic gene regulation.
Chappell, J., Westbrook, A. M., Verosloff, M. and Lucks, J.B.
Nature Communication | 2017 | Link
A Cell-Free Biosensor for Detecting Quorum Sensing Molecules in P. aeruginosa-Infected Respiratory Samples.
Wan, K.Y., Cameron, L., Chappell, J., Jenson, K., Bell, D.J., Kelwick, R., Kopniczky, M., Davies, J.C., Filloux, A. and Freemont, P.S.
ACS Synthetic Biology | 2017 | Link
Pre-2017
Turning It Up to 11: Modular Proteins Amplify RNA Sensors for Sophisticated Circuitry.
Chappell, J., Lucks, J. B.
Cell Systems | 2016 | Link
Improving fold activation of small transcription activating RNAs (STARs) with rational RNA engineering strategies.
Meyer, S., Chappell, J., Sankar, S., Chew, R. and Lucks, J.B.
Biotechnology and bioengineering | 2016 | Link
A renaissance in RNA synthetic biology: new mechanisms, applications and tools for the future.
Chappell, J., Watters, K.E., Takahashi, M.K. and Lucks, J.B.
Current opinion in chemical biology | 2015 | Link
Characterizing and prototyping genetic networks with cell-free transcription-translation reactions.
Takahashi, M.K., Hayes, C.A., Chappell, J., Sun, Z.Z., Murray, R.M., Noireaux, V. and Lucks, J.B.
Methods | 2015 | Link
Rapidly characterizing the fast dynamics of RNA genetic circuitry with cell-free transcription-translation (TX-TL) systems.
Takahashi, M.K., Chappell, J., Hayes, C.A., Sun, Z.Z., Kim, J., Singhal, V., Spring, K.J., Al-Khabouri, S., Fall, C.P., Noireaux, V., Murray, R.M. and Lucks, J.B.
ACS synthetic biology | 2015 | Link | † Featured on the cover.
Creating small transcription activating RNAs.
Chappell, J., Takahashi, M.K. and Lucks, J.B.
Nature chemical biology | 2015 | Link | † Highlighted in Nature Methods (doi:10.1038/nmeth.3354) and in commentary on Science Daily.
The centrality of RNA for engineering gene expression.
Chappell, J., Takahashi, M.K., Meyer, S., Loughrey, D., Watters, K.E. and Lucks, J.B.
Biotechnology journal | 2013 | Link
Validation of an entirely in vitro approach for rapid prototyping of DNA regulatory elements for synthetic biology.
Chappell, J., Jensen, K. and Freemont, P.S.
Nucleic acids research | 2013 | Link
In vivo and in vitro characterization of sigma70 constitutive promoters by real-time PCR and fluorescent measurements.
Chappell, J. and Freemont, P.S.
Methods in molecular biology | 2013 | Link
Synthetic biology – A new generation of biofilm biosensors.
Chappell, J. and Freemont
The Science and Applications of Synthetic and Systems Biology | 2011 | Link
Opportunities for microfluidic technologies in synthetic biology.
Gulati, S., Rouilly, V., Niu, X., Chappell, J., Kitney, R.I., Edel, J.B., Freemont, P.S. and deMello, A.J
Journal of the Royal Society interface | 2009 | Link
2022
RNA Compensation: A Positive Feedback Insulation Strategy for RNA-Based Transcription Networks
Liu,B., Samaniego, C, C., Bennett, M., Chappell, J.*, and Franco, E.*
ACS Synthetic Biology | 2022| Link
A split ribozyme that links detection of a native RNA to orthogonal protein outputs.
Gambill, L., Staubus, A., Ameruoso, A., and Chappell, J. *
Submitted | 2022 | Preprint
Uncovering the distinct properties of a bacterial type I-E CRISPR activation system
Villegas Kcam, M.V., and Chappell, J. *
ACS Synthetic Biology | 2022 | Link
2021
Activating natural product synthesis using CRISPR interference and activation systems in Streptomyces.
Ameruoso, A., Villegas Kcam, M.V., Cohen K.P., and Chappell, J. *
Submitted | 2021 | Preprint
Computational design of Small Transcription Activating RNAs (STARs)
Liu, B. and Chappell, J.*
Methods in molecular biology, submitted | 2021
Design, characterization, and application of targeted gene activation in bacteria using a modular CRISPRa system
Villegas Kcam, M.V., and Chappell, J.*
Methods in molecular biology, submitted | 2021
Rational engineering of a modular and target flexible bacterial CRISPR-Cas activation platform.
Villegas Kcam, M.V., Tsong, A. and Chappell, J.*
Nucleic Acids Research | 2021 | Link
2019
Brave new ‘RNA’ world—Advances in RNA tools and their application for understanding and engineering biological systems.
Ameruoso, A., Liu, B., Villegas Kcam, M.V., Gambill, L., Chappell, J. *
Current Opinion in Systems Biology | 2019 | Link
A synthetic system for asymmetric cell division in Escherichia coli.
Molinari, S., Shis, D.L., Bhakta, S.P., Chappell, J., Igoshin, O.A., Bennett, M.
Nature Chemical Biology | 2019 | Link
Distinct timescales of RNA regulators enable the construction of a genetic pulse generator.
Westbrook, A., Tang, X., Marhsall, R., Maxwell, C., Chappell, J., Agrawal, D., Dunlop, M., Noireaux, V., Beisel, C., Lucks, J. B., Franco, E. M.
Biotechnology and Bioengineering | 2019 | Link
PLANT-DX: A Molecular Diagnostic for Point of Use Detection of Plant Pathogens.
Verosloff, M., Chappell, J., Perry, K., Thompson, J., Lucks, J.B.
ACS Synthetic Biology | 2019 | Link
2017
Computational design of Small Transcription Activating RNAs (STARs) for versatile and dynamic gene regulation.
Chappell, J., Westbrook, A. M., Verosloff, M. and Lucks, J.B.
Nature Communication | 2017 | Link
A Cell-Free Biosensor for Detecting Quorum Sensing Molecules in P. aeruginosa-Infected Respiratory Samples.
Wan, K.Y., Cameron, L., Chappell, J., Jenson, K., Bell, D.J., Kelwick, R., Kopniczky, M., Davies, J.C., Filloux, A. and Freemont, P.S.
ACS Synthetic Biology | 2017 | Link
Pre-2017
Turning It Up to 11: Modular Proteins Amplify RNA Sensors for Sophisticated Circuitry.
Chappell, J., Lucks, J. B.
Cell Systems | 2016 | Link
Improving fold activation of small transcription activating RNAs (STARs) with rational RNA engineering strategies.
Meyer, S., Chappell, J., Sankar, S., Chew, R. and Lucks, J.B.
Biotechnology and bioengineering | 2016 | Link
A renaissance in RNA synthetic biology: new mechanisms, applications and tools for the future.
Chappell, J., Watters, K.E., Takahashi, M.K. and Lucks, J.B.
Current opinion in chemical biology | 2015 | Link
Characterizing and prototyping genetic networks with cell-free transcription-translation reactions.
Takahashi, M.K., Hayes, C.A., Chappell, J., Sun, Z.Z., Murray, R.M., Noireaux, V. and Lucks, J.B.
Methods | 2015 | Link
Rapidly characterizing the fast dynamics of RNA genetic circuitry with cell-free transcription-translation (TX-TL) systems.
Takahashi, M.K., Chappell, J., Hayes, C.A., Sun, Z.Z., Kim, J., Singhal, V., Spring, K.J., Al-Khabouri, S., Fall, C.P., Noireaux, V., Murray, R.M. and Lucks, J.B.
ACS synthetic biology | 2015 | Link | † Featured on the cover.
Creating small transcription activating RNAs.
Chappell, J., Takahashi, M.K. and Lucks, J.B.
Nature chemical biology | 2015 | Link | † Highlighted in Nature Methods (doi:10.1038/nmeth.3354) and in commentary on Science Daily.
The centrality of RNA for engineering gene expression.
Chappell, J., Takahashi, M.K., Meyer, S., Loughrey, D., Watters, K.E. and Lucks, J.B.
Biotechnology journal | 2013 | Link
Validation of an entirely in vitro approach for rapid prototyping of DNA regulatory elements for synthetic biology.
Chappell, J., Jensen, K. and Freemont, P.S.
Nucleic acids research | 2013 | Link
In vivo and in vitro characterization of sigma70 constitutive promoters by real-time PCR and fluorescent measurements.
Chappell, J. and Freemont, P.S.
Methods in molecular biology | 2013 | Link
Synthetic biology – A new generation of biofilm biosensors.
Chappell, J. and Freemont
The Science and Applications of Synthetic and Systems Biology | 2011 | Link
Opportunities for microfluidic technologies in synthetic biology.
Gulati, S., Rouilly, V., Niu, X., Chappell, J., Kitney, R.I., Edel, J.B., Freemont, P.S. and deMello, A.J
Journal of the Royal Society interface | 2009 | Link