Publications:

• Villegas Kcam, M.V., Tsong, A. and Chappell, J. Rational engineering of a modular and target flexible bacterial CRISPR-Cas activation platform. ,2020. Submitted.

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• A Ameruoso, L Gambill, B Liu, MC Villegas Kcam, J Chappell. Brave new ‘RNA’ world—advances in RNA tools and their application for understanding and engineering biological systems. ​​Current Opinion in Systems Biology, 2019. Link to article

• Molinari, S., Shis, D.L., Bhakta, S.P., Chappell, J., Igoshin, O.A., Bennett, M. (2019) A synthetic system for asymmetric cell division in Escherichia coli.. Nature Chemical Biology, 15, 917–924.  Link to article

 

• Westbrook, A., Tang, X., Marhsall, R., Maxwell, C., Chappell, J., Agrawal, D., Dunlop, M., Noireaux, V., Beisel, C., Lucks, J. B., Franco, E. M. (2019) "Distinct timescales of RNA regulators enable the construction of a genetic pulse generator." Biotechnology and Bioengineering, 116, 1139-1151. Link to article

 

• Verosloff, M., Chappell, J., Perry, K., Thompson, J., Lucks, J.B. (2018). PLANT-DX: A Molecular Diagnostic for Point of Use Detection of Plant Pathogens. ACS Synthetic Biology, 8, 902-905. Link to article

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• KY Wen, L Cameron, J Chappell, K Jensen, D J Bell, R Kelwick, M Kopniczky, JC Davies, A Filloux, PS Freemont. A Cell-Free Biosensor for Detecting Quorum Sensing Molecules in P. aeruginosa-Infected Respiratory Samples​. ACS Synthetic Biology, 2017. Link to article. 

 

• J Chappell, A Westbrook, M Verosloff, JB Lucks. Computational design of Small Transcription Activating RNAs (STARs) for versatile and dynamic gene regulation. Nature communications, 2017. Link to article. 

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• J Chappell, JB Lucks. Turning It Up to 11: Modular Proteins Amplify RNA Sensors for Sophisticated Circuitry. Cell Systems, 2016. Link to article.

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• S Meyer, J Chappell, S Sankar, R Chew, JB Lucks. Improving fold activation of small transcription activating RNAs (STARs) with rational RNA engineering strategies. Biotechnology and bioengineering, 2015. ​Link to article.

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• J Chappell, KE Watters, MK Takahashi, JB Lucks. A renaissance in RNA synthetic biology: new mechanisms, applications and tools for the future. Current opinion in chemical biology, 2015. Link to article.

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• MK Takahashi, CA Hayes, J Chappell, ZZ Sun, RM Murray, V Nouireaux, JB Lucks. Characterizing and prototyping genetic networks with cell-free transcription–translation reactions. Methods, 2015. Link to article.

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• J Chappell, MK Takahashi, JB Lucks. Creating small transcription activating RNAs. Nature chemical biology, 2015. Link to article.

 

• MK Takashashi, J Chappell, CA Hayes, ZZ Sun, J Kim, V  Singhal, KJ Spring, S Al-Khabouri, CP Fall, V Noireaux, RM Murray, JB Lucks. Rapidly characterizing the fast dynamics of RNA genetic circuitry with cell-free transcription–translation (TX-TL) systems. ACS synthetic biology, 2014. Link to article. 

 

• J Chappell, MK Takahashi, S Meyer, D Loughrey, KE Watters, JB Lucks. The centrality of RNA for engineering gene expression. Biotechnology journal, 2013. Link to article. 

 

• J Chappell, K Jensen, PS Freemont. Validation of an entirely in vitro approach for rapid prototyping of DNA regulatory elements for synthetic biology. Nucleic acids research, 2013. Link to article.

 

• J Chappell, P Freemont. In Vivo and In Vitro Characterization of σ 70 Constitutive Promoters by Real-Time PCR and Fluorescent Measurements. Synthetic Biology (Methods in Molecular Biology 1073), 2013. Link to article.

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• S Gulati, V Rouilly, X Niu, J Chappell, RI Kitney, JB Edel, PS Freemont. Opportunities for microfluidic technologies in synthetic biology. Journal of The Royal Society Interface, 2009. Link to article.