PUBLICATIONS
Computational design of Small Transcription Activating RNAs (STARs)
B Liu and J Chappell.
Methods in Molecular Biology | 2021 | Submitted
Design, characterization, and application of targeted gene activation in bacteria using a modular CRISPRa system.
MC Villegas Kcam and J Chappell.
Methods in Molecular Biology | 2021 Submitted.
Rational engineering of a modular and target flexible bacterial CRISPR-Cas activation platform.
MC Villegas Kcam, A Tsong, and J Chappell
Nucleic Acids Research | 2021 | LINK
Brave new 'RNA' world - advances in tools and their application for understanding and engineering biological systems.
A Ameruoso, L Gambill, B Liu, MC Villegas Kcam, J Chappell
Current Opinion in Systems Biology | 2019 | LINK
A synthetic system for asymmetric cell division in Escherichia coli.
S Molinari, DL Shis, SP Bhakta, J Chappell, OA Igoshin, MR Bennett
Nature Chemical Biology | 2019 | LINK
Distinct timescales of RNA regulators enable the construction of a genetic pulse generator.
A Westbrook, X Tang, R Marshall, CS Maxwell, J Chappell, DK Agrawal, MJ Dunlop, V Noireaux, CL Bleisel, JB Lucks, E Franco
Biotechnology and Bioengineering | 2019 | LINK
PLANT-Dx: A molecular diagnostic for point-of-use detection of plant pathogens.
M Verosloff, J Chappell, KL Perry. JR Thompson, JB Lucks
ACS Synthetic Biology | 2019 | LINK
A cell-free biosensor for detecting quorum sensing molecules in P. aeruginosa-infected respiratory samples.
K Yan Wen, L Cameron, J Chappell, K Jensen, DJ Bell, R Kelwick, M Kopniczky, JC Davies, A Filloux, PS Freemont
ACS Synthetic Biology | 2017 | LINK
Computational design of small transcription activating RNAs for versatile and dynamic gene regulation.
J Chappell, A Westbrook, M Verosloff, JB Lucks
Nature Communications | 2017 | LINK
Tuning it up to 11: modular proteins amplify RNA sensors for sophisticated circuitry.
J Chappell, JB Lucks
Cell Systems | 2016 | LINK
Improving fold activation of small transcription activating RNAs (STARs) with rational RNA engineering strategies.
S Meyer, J Chappell, S Sankar, R Chew, JB Lucks
Biotechnology and Bioengineering | 2015 | LINK
A renaissance in RNA synthetic biology: new mechanisms, applications and tools for the future.
J Chappell, KE Watters, MK Takahashi, JB Lucks
Current Opinion in Chemical Biology | 2015 | LINK
Characterizing and prototyping genetic networks with cell-free transcription-translation reactions.
MK Takahashi, CA Hayes, J Chappell, ZZ Sun, RM Murray, V Noireaux, JB Lucks
Methods | 2015 | LINK
Creating small transcription activating RNAs.
J Chappell, MK Takahashi, JB Lucks
Nature Chemical Biology | 2015 | LINK
Rapidly characterizing the fast dynamics of RNA genetic circuitry with cell-free transcription-translation (TX-TL) systems
MK Takahashi, J Chappell, CA Hayes, ZZ Sun, J Kim, V Singhal, KJ Spring, S Al-Khabouri, CP Fall, V Noireaux, RM Murray, JB Lucks
ACS Synthetic Biology | 2014 | LINK
The centrality of RNA for engineering gene expression.
J Chappell, MK Takahashi, S Meyer, D Loughrey, KE Watters, JB Lucks
Biotechnology Journal | 2013 | LINK
Validation of an entirely in vitro approach for rapid prototyping of DNA regulatory elements for synthetic biology.
J Chappell, K Jensen, PS Freemont
Nucleic Acids Research | 2013 | LINK
In vivo and in vitro characterization of σ70 constitutive promoters by real-time PCR and fluorescent measurements.
J Chappell, PS Freemont
Synthetic Biology (Methods in Molecular Biology, 1073) | 2013 | LINK
Opportunities for microfluidic technologies in synthetic biology.
S Gulati, V Rouilly, X Niu, J Chappell, RI Kitney, JB Edel, PS Freemont, AJ deMello
Journal of The Royal Society Interface | 2009 | LINK
B Liu and J Chappell.
Methods in Molecular Biology | 2021 | Submitted
Design, characterization, and application of targeted gene activation in bacteria using a modular CRISPRa system.
MC Villegas Kcam and J Chappell.
Methods in Molecular Biology | 2021 Submitted.
Rational engineering of a modular and target flexible bacterial CRISPR-Cas activation platform.
MC Villegas Kcam, A Tsong, and J Chappell
Nucleic Acids Research | 2021 | LINK
Brave new 'RNA' world - advances in tools and their application for understanding and engineering biological systems.
A Ameruoso, L Gambill, B Liu, MC Villegas Kcam, J Chappell
Current Opinion in Systems Biology | 2019 | LINK
A synthetic system for asymmetric cell division in Escherichia coli.
S Molinari, DL Shis, SP Bhakta, J Chappell, OA Igoshin, MR Bennett
Nature Chemical Biology | 2019 | LINK
Distinct timescales of RNA regulators enable the construction of a genetic pulse generator.
A Westbrook, X Tang, R Marshall, CS Maxwell, J Chappell, DK Agrawal, MJ Dunlop, V Noireaux, CL Bleisel, JB Lucks, E Franco
Biotechnology and Bioengineering | 2019 | LINK
PLANT-Dx: A molecular diagnostic for point-of-use detection of plant pathogens.
M Verosloff, J Chappell, KL Perry. JR Thompson, JB Lucks
ACS Synthetic Biology | 2019 | LINK
A cell-free biosensor for detecting quorum sensing molecules in P. aeruginosa-infected respiratory samples.
K Yan Wen, L Cameron, J Chappell, K Jensen, DJ Bell, R Kelwick, M Kopniczky, JC Davies, A Filloux, PS Freemont
ACS Synthetic Biology | 2017 | LINK
Computational design of small transcription activating RNAs for versatile and dynamic gene regulation.
J Chappell, A Westbrook, M Verosloff, JB Lucks
Nature Communications | 2017 | LINK
Tuning it up to 11: modular proteins amplify RNA sensors for sophisticated circuitry.
J Chappell, JB Lucks
Cell Systems | 2016 | LINK
Improving fold activation of small transcription activating RNAs (STARs) with rational RNA engineering strategies.
S Meyer, J Chappell, S Sankar, R Chew, JB Lucks
Biotechnology and Bioengineering | 2015 | LINK
A renaissance in RNA synthetic biology: new mechanisms, applications and tools for the future.
J Chappell, KE Watters, MK Takahashi, JB Lucks
Current Opinion in Chemical Biology | 2015 | LINK
Characterizing and prototyping genetic networks with cell-free transcription-translation reactions.
MK Takahashi, CA Hayes, J Chappell, ZZ Sun, RM Murray, V Noireaux, JB Lucks
Methods | 2015 | LINK
Creating small transcription activating RNAs.
J Chappell, MK Takahashi, JB Lucks
Nature Chemical Biology | 2015 | LINK
Rapidly characterizing the fast dynamics of RNA genetic circuitry with cell-free transcription-translation (TX-TL) systems
MK Takahashi, J Chappell, CA Hayes, ZZ Sun, J Kim, V Singhal, KJ Spring, S Al-Khabouri, CP Fall, V Noireaux, RM Murray, JB Lucks
ACS Synthetic Biology | 2014 | LINK
The centrality of RNA for engineering gene expression.
J Chappell, MK Takahashi, S Meyer, D Loughrey, KE Watters, JB Lucks
Biotechnology Journal | 2013 | LINK
Validation of an entirely in vitro approach for rapid prototyping of DNA regulatory elements for synthetic biology.
J Chappell, K Jensen, PS Freemont
Nucleic Acids Research | 2013 | LINK
In vivo and in vitro characterization of σ70 constitutive promoters by real-time PCR and fluorescent measurements.
J Chappell, PS Freemont
Synthetic Biology (Methods in Molecular Biology, 1073) | 2013 | LINK
Opportunities for microfluidic technologies in synthetic biology.
S Gulati, V Rouilly, X Niu, J Chappell, RI Kitney, JB Edel, PS Freemont, AJ deMello
Journal of The Royal Society Interface | 2009 | LINK