PUBLICATIONS
*Corresponding author(s)
2021
Activating natural product synthesis using CRISPR interference and activation systems in Streptomyces.
Ameruoso, A,, Villegas Kcam, M.V., Cohen K.P., and Chappell, J. *
Submitted | 2021 | Preprint
RNA compensation: A positive feedback insulation strategy for RNA-based networks
Liu, B., Cuba Samaniego, C., Bennett, B.R., Chappell, J.* and Franco E*
Submitted | 2021 | Preprint
Uncovering the distinct properties of a bacterial type I-E CRISPR activation system
Villegas Kcam, M.V., and Chappell, J. *
Submitted | 2021 | Preprint
Computational design of Small Transcription Activating RNAs (STARs)
Liu, B. and Chappell, J.*
Methods in molecular biology, submitted | 2021
Design, characterization, and application of targeted gene activation in bacteria using a modular CRISPRa system
Villegas Kcam, M.V., and Chappell, J.*
Methods in molecular biology, submitted | 2021
Rational engineering of a modular and target flexible bacterial CRISPR-Cas activation platform.
Villegas Kcam, M.V., Tsong, A. and Chappell, J.*
Nucleic Acids Research | 2021 | Link
2019
Brave new ‘RNA’ world—Advances in RNA tools and their application for understanding and engineering biological systems.
Ameruoso, A., Liu, B., Villegas Kcam, M.V., Gambill, L., Chappell, J. *
Current Opinion in Systems Biology | 2019 | Link
A synthetic system for asymmetric cell division in Escherichia coli.
Molinari, S., Shis, D.L., Bhakta, S.P., Chappell, J., Igoshin, O.A., Bennett, M.
Nature Chemical Biology | 2019 | Link
Distinct timescales of RNA regulators enable the construction of a genetic pulse generator.
Westbrook, A., Tang, X., Marhsall, R., Maxwell, C., Chappell, J., Agrawal, D., Dunlop, M., Noireaux, V., Beisel, C., Lucks, J. B., Franco, E. M.
Biotechnology and Bioengineering | 2019 | Link
PLANT-DX: A Molecular Diagnostic for Point of Use Detection of Plant Pathogens.
Verosloff, M., Chappell, J., Perry, K., Thompson, J., Lucks, J.B.
ACS Synthetic Biology | 2019 | Link
2017
Computational design of Small Transcription Activating RNAs (STARs) for versatile and dynamic gene regulation.
Chappell, J., Westbrook, A. M., Verosloff, M. and Lucks, J.B.
Nature Communication | 2017 | Link
A Cell-Free Biosensor for Detecting Quorum Sensing Molecules in P. aeruginosa-Infected Respiratory Samples.
Wan, K.Y., Cameron, L., Chappell, J., Jenson, K., Bell, D.J., Kelwick, R., Kopniczky, M., Davies, J.C., Filloux, A. and Freemont, P.S.
ACS Synthetic Biology | 2017 | Link
Pre-2017
Turning It Up to 11: Modular Proteins Amplify RNA Sensors for Sophisticated Circuitry.
Chappell, J., Lucks, J. B.
Cell Systems | 2016 | Link
Improving fold activation of small transcription activating RNAs (STARs) with rational RNA engineering strategies.
Meyer, S., Chappell, J., Sankar, S., Chew, R. and Lucks, J.B.
Biotechnology and bioengineering | 2016 | Link
A renaissance in RNA synthetic biology: new mechanisms, applications and tools for the future.
Chappell, J., Watters, K.E., Takahashi, M.K. and Lucks, J.B.
Current opinion in chemical biology | 2015| Link
Characterizing and prototyping genetic networks with cell-free transcription-translation reactions.
Takahashi, M.K., Hayes, C.A., Chappell, J., Sun, Z.Z., Murray, R.M., Noireaux, V. and Lucks, J.B.
Methods | 2015 | Link
Rapidly characterizing the fast dynamics of RNA genetic circuitry with cell-free transcription-translation (TX-TL) systems.
Takahashi, M.K., Chappell, J., Hayes, C.A., Sun, Z.Z., Kim, J., Singhal, V., Spring, K.J., Al-Khabouri, S., Fall, C.P., Noireaux, V., Murray, R.M. and Lucks, J.B.
ACS synthetic biology | 2015 | Link | † Featured on the cover.
Creating small transcription activating RNAs.
Chappell, J., Takahashi, M.K. and Lucks, J.B.
Nature chemical biology | 2015 | Link | † Highlighted in Nature Methods (doi:10.1038/nmeth.3354) and in commentary on Science Daily.
The centrality of RNA for engineering gene expression.
Chappell, J., Takahashi, M.K., Meyer, S., Loughrey, D., Watters, K.E. and Lucks, J.B.
Biotechnology journal | 2013 | Link
Validation of an entirely in vitro approach for rapid prototyping of DNA regulatory elements for synthetic biology.
Chappell, J., Jensen, K. and Freemont, P.S.
Nucleic acids research | 2013 | Link
In vivo and in vitro characterization of sigma70 constitutive promoters by real-time PCR and fluorescent measurements.
Chappell, J. and Freemont, P.S.
| Methods in molecular biology | 2013 | Link
Synthetic biology – A new generation of biofilm biosensors.
Chappell, J. and Freemont
| The Science and Applications of Synthetic and Systems Biology | 2011 | Link
Opportunities for microfluidic technologies in synthetic biology.
Gulati, S., Rouilly, V., Niu, X., Chappell, J., Kitney, R.I., Edel, J.B., Freemont, P.S. and deMello, A.J
| Journal of the Royal Society interface | 2009 | Link
2021
Activating natural product synthesis using CRISPR interference and activation systems in Streptomyces.
Ameruoso, A,, Villegas Kcam, M.V., Cohen K.P., and Chappell, J. *
Submitted | 2021 | Preprint
RNA compensation: A positive feedback insulation strategy for RNA-based networks
Liu, B., Cuba Samaniego, C., Bennett, B.R., Chappell, J.* and Franco E*
Submitted | 2021 | Preprint
Uncovering the distinct properties of a bacterial type I-E CRISPR activation system
Villegas Kcam, M.V., and Chappell, J. *
Submitted | 2021 | Preprint
Computational design of Small Transcription Activating RNAs (STARs)
Liu, B. and Chappell, J.*
Methods in molecular biology, submitted | 2021
Design, characterization, and application of targeted gene activation in bacteria using a modular CRISPRa system
Villegas Kcam, M.V., and Chappell, J.*
Methods in molecular biology, submitted | 2021
Rational engineering of a modular and target flexible bacterial CRISPR-Cas activation platform.
Villegas Kcam, M.V., Tsong, A. and Chappell, J.*
Nucleic Acids Research | 2021 | Link
2019
Brave new ‘RNA’ world—Advances in RNA tools and their application for understanding and engineering biological systems.
Ameruoso, A., Liu, B., Villegas Kcam, M.V., Gambill, L., Chappell, J. *
Current Opinion in Systems Biology | 2019 | Link
A synthetic system for asymmetric cell division in Escherichia coli.
Molinari, S., Shis, D.L., Bhakta, S.P., Chappell, J., Igoshin, O.A., Bennett, M.
Nature Chemical Biology | 2019 | Link
Distinct timescales of RNA regulators enable the construction of a genetic pulse generator.
Westbrook, A., Tang, X., Marhsall, R., Maxwell, C., Chappell, J., Agrawal, D., Dunlop, M., Noireaux, V., Beisel, C., Lucks, J. B., Franco, E. M.
Biotechnology and Bioengineering | 2019 | Link
PLANT-DX: A Molecular Diagnostic for Point of Use Detection of Plant Pathogens.
Verosloff, M., Chappell, J., Perry, K., Thompson, J., Lucks, J.B.
ACS Synthetic Biology | 2019 | Link
2017
Computational design of Small Transcription Activating RNAs (STARs) for versatile and dynamic gene regulation.
Chappell, J., Westbrook, A. M., Verosloff, M. and Lucks, J.B.
Nature Communication | 2017 | Link
A Cell-Free Biosensor for Detecting Quorum Sensing Molecules in P. aeruginosa-Infected Respiratory Samples.
Wan, K.Y., Cameron, L., Chappell, J., Jenson, K., Bell, D.J., Kelwick, R., Kopniczky, M., Davies, J.C., Filloux, A. and Freemont, P.S.
ACS Synthetic Biology | 2017 | Link
Pre-2017
Turning It Up to 11: Modular Proteins Amplify RNA Sensors for Sophisticated Circuitry.
Chappell, J., Lucks, J. B.
Cell Systems | 2016 | Link
Improving fold activation of small transcription activating RNAs (STARs) with rational RNA engineering strategies.
Meyer, S., Chappell, J., Sankar, S., Chew, R. and Lucks, J.B.
Biotechnology and bioengineering | 2016 | Link
A renaissance in RNA synthetic biology: new mechanisms, applications and tools for the future.
Chappell, J., Watters, K.E., Takahashi, M.K. and Lucks, J.B.
Current opinion in chemical biology | 2015| Link
Characterizing and prototyping genetic networks with cell-free transcription-translation reactions.
Takahashi, M.K., Hayes, C.A., Chappell, J., Sun, Z.Z., Murray, R.M., Noireaux, V. and Lucks, J.B.
Methods | 2015 | Link
Rapidly characterizing the fast dynamics of RNA genetic circuitry with cell-free transcription-translation (TX-TL) systems.
Takahashi, M.K., Chappell, J., Hayes, C.A., Sun, Z.Z., Kim, J., Singhal, V., Spring, K.J., Al-Khabouri, S., Fall, C.P., Noireaux, V., Murray, R.M. and Lucks, J.B.
ACS synthetic biology | 2015 | Link | † Featured on the cover.
Creating small transcription activating RNAs.
Chappell, J., Takahashi, M.K. and Lucks, J.B.
Nature chemical biology | 2015 | Link | † Highlighted in Nature Methods (doi:10.1038/nmeth.3354) and in commentary on Science Daily.
The centrality of RNA for engineering gene expression.
Chappell, J., Takahashi, M.K., Meyer, S., Loughrey, D., Watters, K.E. and Lucks, J.B.
Biotechnology journal | 2013 | Link
Validation of an entirely in vitro approach for rapid prototyping of DNA regulatory elements for synthetic biology.
Chappell, J., Jensen, K. and Freemont, P.S.
Nucleic acids research | 2013 | Link
In vivo and in vitro characterization of sigma70 constitutive promoters by real-time PCR and fluorescent measurements.
Chappell, J. and Freemont, P.S.
| Methods in molecular biology | 2013 | Link
Synthetic biology – A new generation of biofilm biosensors.
Chappell, J. and Freemont
| The Science and Applications of Synthetic and Systems Biology | 2011 | Link
Opportunities for microfluidic technologies in synthetic biology.
Gulati, S., Rouilly, V., Niu, X., Chappell, J., Kitney, R.I., Edel, J.B., Freemont, P.S. and deMello, A.J
| Journal of the Royal Society interface | 2009 | Link